The roles of plant dsRNA-binding proteins in RNAi-like pathways

Shaun Curtin, John M. Watson, Neil A. Smith, Andrew L. Eamens, Christopher Blanchard, Peter M. Waterhouse

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Dicers are associated with double-stranded RNA-binding proteins (dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi)RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway.
Original languageEnglish
Pages (from-to)2753-2760
Number of pages8
JournalFEBS Letters
Volume582
Issue number18
DOIs
Publication statusPublished - 2008

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RNA-Binding Proteins
RNA Interference
Carrier Proteins
Dichlororibofuranosylbenzimidazole
Viral RNA
MicroRNAs
Arabidopsis
Small Interfering RNA
Redundancy
Animals
Proteins

Cite this

Curtin, S., Watson, J. M., Smith, N. A., Eamens, A. L., Blanchard, C., & Waterhouse, P. M. (2008). The roles of plant dsRNA-binding proteins in RNAi-like pathways. FEBS Letters, 582(18), 2753-2760. https://doi.org/10.1016/j.febslet.2008.07.004
Curtin, Shaun ; Watson, John M. ; Smith, Neil A. ; Eamens, Andrew L. ; Blanchard, Christopher ; Waterhouse, Peter M. / The roles of plant dsRNA-binding proteins in RNAi-like pathways. In: FEBS Letters. 2008 ; Vol. 582, No. 18. pp. 2753-2760.
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Curtin, S, Watson, JM, Smith, NA, Eamens, AL, Blanchard, C & Waterhouse, PM 2008, 'The roles of plant dsRNA-binding proteins in RNAi-like pathways', FEBS Letters, vol. 582, no. 18, pp. 2753-2760. https://doi.org/10.1016/j.febslet.2008.07.004

The roles of plant dsRNA-binding proteins in RNAi-like pathways. / Curtin, Shaun; Watson, John M.; Smith, Neil A.; Eamens, Andrew L.; Blanchard, Christopher; Waterhouse, Peter M.

In: FEBS Letters, Vol. 582, No. 18, 2008, p. 2753-2760.

Research output: Contribution to journalArticle

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AU - Curtin, Shaun

AU - Watson, John M.

AU - Smith, Neil A.

AU - Eamens, Andrew L.

AU - Blanchard, Christopher

AU - Waterhouse, Peter M.

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AB - Dicers are associated with double-stranded RNA-binding proteins (dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi)RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway.

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KW - Dicers

KW - DsRBPs

KW - MiRNAs

KW - SiRNAs

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