The roles of plant dsRNA-binding proteins in RNAi-like pathways

Shaun Curtin; John M. Watson; Neil A. Smith; Andrew L. Eamens; Christopher Blanchard; Peter M. Waterhouse

doi:10.1016/j.febslet.2008.07.004

The roles of plant dsRNA-binding proteins in RNAi-like pathways

Shaun Curtin, John M. Watson, Neil A. Smith, Andrew L. Eamens, Christopher Blanchard, Peter M. Waterhouse

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

Dicers are associated with double-stranded RNA-binding proteins (dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi)RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway.

Original language	English
Pages (from-to)	2753-2760
Number of pages	8
Journal	FEBS Letters
Volume	582
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2008.07.004
Publication status	Published - 2008

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10.1016/j.febslet.2008.07.004

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title = "The roles of plant dsRNA-binding proteins in RNAi-like pathways",

abstract = "Dicers are associated with double-stranded RNA-binding proteins (dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi)RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway.",

keywords = "Dicer-like, Dicers, DsRBPs, MiRNAs, SiRNAs",

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note = "Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = FEBS Letters. ISSNs: 0014-5793; ",

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T1 - The roles of plant dsRNA-binding proteins in RNAi-like pathways

AU - Curtin, Shaun

AU - Watson, John M.

AU - Smith, Neil A.

AU - Eamens, Andrew L.

AU - Blanchard, Christopher

AU - Waterhouse, Peter M.

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = FEBS Letters. ISSNs: 0014-5793;

PY - 2008

Y1 - 2008

N2 - Dicers are associated with double-stranded RNA-binding proteins (dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi)RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway.

AB - Dicers are associated with double-stranded RNA-binding proteins (dsRBPs) in animals. In the plant, Arabidopsis, there are four dicer-like (DCL) proteins and five potential dsRBPs. These DCLs act redundantly and hierarchically. However, we show there is little or no redundancy or hierarchy amongst the DRBs in their DCL interactions. DCL1 operates exclusively with DRB1 to produce micro (mi)RNAs, DCL4 operates exclusively with DRB4 to produce trans-acting (ta) siRNAs and 21nt siRNAs from viral RNA. DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway.

KW - Dicer-like

KW - Dicers

KW - DsRBPs

KW - MiRNAs

KW - SiRNAs

U2 - 10.1016/j.febslet.2008.07.004

DO - 10.1016/j.febslet.2008.07.004

M3 - Article

SN - 0014-5793

VL - 582

SP - 2753

EP - 2760

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

The roles of plant dsRNA-binding proteins in RNAi-like pathways

Abstract

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