The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration

Louise McGonigle, Angela Mousley, Nikki Marks, Gerard Brennan, John Dalton, Terry Spithill, Tim Day, Aaron Maule

Research output: Contribution to journalArticle

130 Citations (Scopus)
43 Downloads (Pure)

Abstract

Probing protein function in parasitic flatworms is hampered by the difficulties associated with the development of transgenic approaches. Although RNA interference (RNAi) in schistosomes shows much promise, it has not been reported in other trematodes.Here, we show the successful silencing of the cysteine proteases cathepsin B and L in the infective stage of Fasciola hepatica newly excysted juveniles (NEJs). Silencing resulted in marked reductions in target transcript levels and significant diminution in the encoded proteins in the gut. RNAi of either enzyme in NEJs induced transient, abnormal locomotory phenotypes, and significantly reduced penetration of the rat intestinal wall.
Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalInternational Journal for Parasitology
Volume38
Issue number2
DOIs
Publication statusPublished - 2008

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