The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration

Louise McGonigle, Angela Mousley, Nikki Marks, Gerard Brennan, John Dalton, Terry Spithill, Tim Day, Aaron Maule

    Research output: Contribution to journalArticlepeer-review

    148 Citations (Scopus)
    105 Downloads (Pure)

    Abstract

    Probing protein function in parasitic flatworms is hampered by the difficulties associated with the development of transgenic approaches. Although RNA interference (RNAi) in schistosomes shows much promise, it has not been reported in other trematodes.Here, we show the successful silencing of the cysteine proteases cathepsin B and L in the infective stage of Fasciola hepatica newly excysted juveniles (NEJs). Silencing resulted in marked reductions in target transcript levels and significant diminution in the encoded proteins in the gut. RNAi of either enzyme in NEJs induced transient, abnormal locomotory phenotypes, and significantly reduced penetration of the rat intestinal wall.
    Original languageEnglish
    Pages (from-to)149-155
    Number of pages7
    JournalInternational Journal for Parasitology
    Volume38
    Issue number2
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Dive into the research topics of 'The silencing of cysteine proteases in Fasciola hepatica newly excysted juveniles using RNA interference reduces gut penetration'. Together they form a unique fingerprint.

    Cite this