The Synergistic Effect of Taurine and Caffeine on Platelet Activity and Hemostatic Function

Several studies have shown the deleterious effects of energy drinks, containing taurine and caffeine, on cardiachealth. This study aimed to examine synergistic effect of taurine and caffeine on platelets and haemostasis because platelethyperactivity is a known predictor of cardiovascular diseases. Blood from twelve healthy volunteers aged 18 – 60 years wasincubated with 500 µM taurine and 700 µM caffeine individually or together. Platelet activity was evaluated using plateletaggregation assays induced by ADP and collagen, and, haemostatic function by coagulation profile testing, glucose, lipidprofile and inflammation marker C- reactive protein assessment (CRP). The optimal time and doses of taurine and caffeinerequired to inhibit platelet hyperactivity in vitro were established. A combined action of lower doses of taurine and caffeine(T+C) inhibited platelet aggregation, induced by ADP and collagen, greater than taurine or caffeine individually (p < 0.05).Taurine and T+C increased prothrombin clotting time (PT) significantly (p < 0.05), while caffeine alone decreased PT (p <0.05). Caffeine alone increased CRP (p < 0.05). No significant change was observed in lipid parameters. These data supportour hypothesis that, synergistically lower concentrations of taurine and caffeine, found in much higher concentrations inenergy drinks, may be instrumental in reducing platelet activity and prolongation of clot formation.