The use of poly(vinyl alcohol) to cross-link penicillinase for the fabrication of a penicillin potentiometric biosensor

Fatima Ismail, Samuel B. Adeloju

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chemically cross-linked PVA films are permeable matrices for the fabrication of biosensors. PVA provides an attractive immobilisation method as it preserves the enzymatic activity. Penicillinase (P'nase) was cross-linked with poly(vinyl alcohol) (PVA) and bovine serum albumin (BSA). The optimum conditions for the of BSA-PVA-P'nase film were: 2.5% w/v PVA, 0.006% w/v BSA, 2.4mM penicillin (Pen) and 16U/mL P'nase. The minimum detectable concentration was 1.7μM. The linear concentration range obtained for the BSA-PVA film was 7.5-283μM. The BSA-PVA P'nase biosensor detected penicillin in amoxycillin with an average percentage recovery of 97±12%. Higher penicillin concentrations (10-20ppm) were detected more successfully than lower concentrations (≤5ppm). These results indicate that further work is required to enable the successful detection of lower penicillin concentrations such as 5ppm.
Original languageEnglish
Pages (from-to)2701-2709
Number of pages9
JournalElectroanalysis
Volume26
Issue number12
Early online date12 Nov 2014
DOIs
Publication statusPublished - 01 Dec 2014

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