Timing of floral evocation in the grapevine (Vitis Vinifera L. cv. Chardonnay) is identified by cyto-histological changes in the vegetative shoot apical meristem

Christopher Steel, Peter Noyce, C.P.L Grof, C.E Offler

Research output: Contribution to journalArticle

Abstract


Background and Aims

The grapevine floral development pathway commences in early spring with floral evocation in the shoot apical meristem (SAM) of the sylleptic latent bud. Grapevine floral evocation is defined as the irreversible biochemical and cellular changes in the SAM, which commit the plant to the subsequent formation of flowers. These changes are induced by non‐leaf derived stimuli acting directly on the SAM. The timing of floral evocation, however, between budburst and floral initiation, is unknown. The aims of this study were: (i) to establish a precise timing for floral evocation; and (ii) to identify reliable reproducible markers for floral evocation.

Methods and Results

Sylleptic primary latent buds of Vitis vinifera L. cv. Chardonnay were harvested at 9 am and 5 pm daily, from budburst to 5 days after floral initiation. The SAMs were examined first by stereomicroscope dissection to determine the timing of floral initiation, and then by histological sectioning of those SAMs with nil floral initiation, to determine the timing of floral evocation. Floral initiation occurred 43 days after budburst. A classic cyto‐histological zonation pattern of three regions was observed in all vegetative SAMs up to floral evocation, which occurred 32 h prior to floral initiation. At floral evocation, a ‘mantle over core’ cyto‐histological zonation pattern of two regions was observed.

Conclusions

The precise timing of floral evocation has been identified and reliable markers for evocation described.

Significance of the Study

Targeted transcriptomic analysis of the pre‐evocation SAM, to investigate the cohort of genes controlling floral evocation, can now be readily undertaken.
Original languageEnglish
Pages (from-to)252-265
Number of pages13
JournalAustralian Journal of Grape and Wine Research
Volume25
Publication statusPublished - 2019

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shoot meristems
apical meristems
Vitis vinifera
buds
transcriptomics
flowering
flowers
genes
methodology

Cite this

@article{efdc34359e8342e98455efc53602a406,
title = "Timing of floral evocation in the grapevine (Vitis Vinifera L. cv. Chardonnay) is identified by cyto-histological changes in the vegetative shoot apical meristem",
abstract = "Background and AimsThe grapevine floral development pathway commences in early spring with floral evocation in the shoot apical meristem (SAM) of the sylleptic latent bud. Grapevine floral evocation is defined as the irreversible biochemical and cellular changes in the SAM, which commit the plant to the subsequent formation of flowers. These changes are induced by non‐leaf derived stimuli acting directly on the SAM. The timing of floral evocation, however, between budburst and floral initiation, is unknown. The aims of this study were: (i) to establish a precise timing for floral evocation; and (ii) to identify reliable reproducible markers for floral evocation.Methods and ResultsSylleptic primary latent buds of Vitis vinifera L. cv. Chardonnay were harvested at 9 am and 5 pm daily, from budburst to 5 days after floral initiation. The SAMs were examined first by stereomicroscope dissection to determine the timing of floral initiation, and then by histological sectioning of those SAMs with nil floral initiation, to determine the timing of floral evocation. Floral initiation occurred 43 days after budburst. A classic cyto‐histological zonation pattern of three regions was observed in all vegetative SAMs up to floral evocation, which occurred 32 h prior to floral initiation. At floral evocation, a ‘mantle over core’ cyto‐histological zonation pattern of two regions was observed. ConclusionsThe precise timing of floral evocation has been identified and reliable markers for evocation described.Significance of the StudyTargeted transcriptomic analysis of the pre‐evocation SAM, to investigate the cohort of genes controlling floral evocation, can now be readily undertaken.",
keywords = "Floral development, floral evocation, floral initiation, grapevine",
author = "Christopher Steel and Peter Noyce and C.P.L Grof and C.E Offler",
year = "2019",
language = "English",
volume = "25",
pages = "252--265",
journal = "Australian Journal of Grape and Wine Research",
issn = "1322-7130",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Timing of floral evocation in the grapevine (Vitis Vinifera L. cv. Chardonnay) is identified by cyto-histological changes in the vegetative shoot apical meristem

AU - Steel, Christopher

AU - Noyce, Peter

AU - Grof, C.P.L

AU - Offler, C.E

PY - 2019

Y1 - 2019

N2 - Background and AimsThe grapevine floral development pathway commences in early spring with floral evocation in the shoot apical meristem (SAM) of the sylleptic latent bud. Grapevine floral evocation is defined as the irreversible biochemical and cellular changes in the SAM, which commit the plant to the subsequent formation of flowers. These changes are induced by non‐leaf derived stimuli acting directly on the SAM. The timing of floral evocation, however, between budburst and floral initiation, is unknown. The aims of this study were: (i) to establish a precise timing for floral evocation; and (ii) to identify reliable reproducible markers for floral evocation.Methods and ResultsSylleptic primary latent buds of Vitis vinifera L. cv. Chardonnay were harvested at 9 am and 5 pm daily, from budburst to 5 days after floral initiation. The SAMs were examined first by stereomicroscope dissection to determine the timing of floral initiation, and then by histological sectioning of those SAMs with nil floral initiation, to determine the timing of floral evocation. Floral initiation occurred 43 days after budburst. A classic cyto‐histological zonation pattern of three regions was observed in all vegetative SAMs up to floral evocation, which occurred 32 h prior to floral initiation. At floral evocation, a ‘mantle over core’ cyto‐histological zonation pattern of two regions was observed. ConclusionsThe precise timing of floral evocation has been identified and reliable markers for evocation described.Significance of the StudyTargeted transcriptomic analysis of the pre‐evocation SAM, to investigate the cohort of genes controlling floral evocation, can now be readily undertaken.

AB - Background and AimsThe grapevine floral development pathway commences in early spring with floral evocation in the shoot apical meristem (SAM) of the sylleptic latent bud. Grapevine floral evocation is defined as the irreversible biochemical and cellular changes in the SAM, which commit the plant to the subsequent formation of flowers. These changes are induced by non‐leaf derived stimuli acting directly on the SAM. The timing of floral evocation, however, between budburst and floral initiation, is unknown. The aims of this study were: (i) to establish a precise timing for floral evocation; and (ii) to identify reliable reproducible markers for floral evocation.Methods and ResultsSylleptic primary latent buds of Vitis vinifera L. cv. Chardonnay were harvested at 9 am and 5 pm daily, from budburst to 5 days after floral initiation. The SAMs were examined first by stereomicroscope dissection to determine the timing of floral initiation, and then by histological sectioning of those SAMs with nil floral initiation, to determine the timing of floral evocation. Floral initiation occurred 43 days after budburst. A classic cyto‐histological zonation pattern of three regions was observed in all vegetative SAMs up to floral evocation, which occurred 32 h prior to floral initiation. At floral evocation, a ‘mantle over core’ cyto‐histological zonation pattern of two regions was observed. ConclusionsThe precise timing of floral evocation has been identified and reliable markers for evocation described.Significance of the StudyTargeted transcriptomic analysis of the pre‐evocation SAM, to investigate the cohort of genes controlling floral evocation, can now be readily undertaken.

KW - Floral development

KW - floral evocation

KW - floral initiation

KW - grapevine

UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/ajgw.12391

M3 - Article

VL - 25

SP - 252

EP - 265

JO - Australian Journal of Grape and Wine Research

JF - Australian Journal of Grape and Wine Research

SN - 1322-7130

ER -