Tumor size as a predictor of lymphatic invasion activity in canine oral melanomas

Kenneth Carroll, Jane Heller, Andrew Peters, Randi Rotne, Andrew Dunn, Charles Kuntz

Research output: Contribution to journalArticle

Abstract

Objective: To investigate putative associations between oral melanoma size and parameters of histological grade such as mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation. Design: Retrospective case series Samples: 59 samples of canine oral melanoma sourced from six diagnostic laboratories within Australia. Procedures: The size of each melanoma was microscopically measured, and each sample was evaluated for parameters of histological grade including mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation, by a veterinary pathologist. The association between tumor size and histological outcomes was then explored using a variety of statistical analyses. Results: A significant relationship was identified between the size of oral melanomas and one histological parameter of grade: lymphatic invasion; with larger tumors, more likely to show invasion (P = 0.023), and further analysis revealed two applicable size thresholds for different clinical scenarios. Lymphatic invasion can confidently be ruled out for tumors <6.5 mm (100% sensitivity), and ruled in for tumors >24.5 mm (100% specificity) in diameter. Conclusions and clinical relevance: The two thresholds developed for lymphatic invasion provide fast, easy and clinically useful recommendations that allow for the early prognostication of oral melanomas based on clinical size.
Original languageEnglish
JournalJournal of the American Veterinary Medical Association
Publication statusAccepted/In press - 2019

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melanoma
Canidae
Melanoma
mouth
neoplasms
dogs
Mitotic Index
Pigmentation
Neoplasms
pigmentation
sampling

Cite this

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title = "Tumor size as a predictor of lymphatic invasion activity in canine oral melanomas",
abstract = "Objective: To investigate putative associations between oral melanoma size and parameters of histological grade such as mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation. Design: Retrospective case series Samples: 59 samples of canine oral melanoma sourced from six diagnostic laboratories within Australia. Procedures: The size of each melanoma was microscopically measured, and each sample was evaluated for parameters of histological grade including mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation, by a veterinary pathologist. The association between tumor size and histological outcomes was then explored using a variety of statistical analyses. Results: A significant relationship was identified between the size of oral melanomas and one histological parameter of grade: lymphatic invasion; with larger tumors, more likely to show invasion (P = 0.023), and further analysis revealed two applicable size thresholds for different clinical scenarios. Lymphatic invasion can confidently be ruled out for tumors <6.5 mm (100{\%} sensitivity), and ruled in for tumors >24.5 mm (100{\%} specificity) in diameter. Conclusions and clinical relevance: The two thresholds developed for lymphatic invasion provide fast, easy and clinically useful recommendations that allow for the early prognostication of oral melanomas based on clinical size.",
author = "Kenneth Carroll and Jane Heller and Andrew Peters and Randi Rotne and Andrew Dunn and Charles Kuntz",
year = "2019",
language = "English",
journal = "Journal of the American Veterinary Medical Association",
issn = "0003-1488",
publisher = "American Veterinary Medical Association",

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TY - JOUR

T1 - Tumor size as a predictor of lymphatic invasion activity in canine oral melanomas

AU - Carroll, Kenneth

AU - Heller, Jane

AU - Peters, Andrew

AU - Rotne, Randi

AU - Dunn, Andrew

AU - Kuntz, Charles

PY - 2019

Y1 - 2019

N2 - Objective: To investigate putative associations between oral melanoma size and parameters of histological grade such as mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation. Design: Retrospective case series Samples: 59 samples of canine oral melanoma sourced from six diagnostic laboratories within Australia. Procedures: The size of each melanoma was microscopically measured, and each sample was evaluated for parameters of histological grade including mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation, by a veterinary pathologist. The association between tumor size and histological outcomes was then explored using a variety of statistical analyses. Results: A significant relationship was identified between the size of oral melanomas and one histological parameter of grade: lymphatic invasion; with larger tumors, more likely to show invasion (P = 0.023), and further analysis revealed two applicable size thresholds for different clinical scenarios. Lymphatic invasion can confidently be ruled out for tumors <6.5 mm (100% sensitivity), and ruled in for tumors >24.5 mm (100% specificity) in diameter. Conclusions and clinical relevance: The two thresholds developed for lymphatic invasion provide fast, easy and clinically useful recommendations that allow for the early prognostication of oral melanomas based on clinical size.

AB - Objective: To investigate putative associations between oral melanoma size and parameters of histological grade such as mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation. Design: Retrospective case series Samples: 59 samples of canine oral melanoma sourced from six diagnostic laboratories within Australia. Procedures: The size of each melanoma was microscopically measured, and each sample was evaluated for parameters of histological grade including mitotic index, nuclear atypia, junctional activity, ulceration, lymphatic invasion and degree of pigmentation, by a veterinary pathologist. The association between tumor size and histological outcomes was then explored using a variety of statistical analyses. Results: A significant relationship was identified between the size of oral melanomas and one histological parameter of grade: lymphatic invasion; with larger tumors, more likely to show invasion (P = 0.023), and further analysis revealed two applicable size thresholds for different clinical scenarios. Lymphatic invasion can confidently be ruled out for tumors <6.5 mm (100% sensitivity), and ruled in for tumors >24.5 mm (100% specificity) in diameter. Conclusions and clinical relevance: The two thresholds developed for lymphatic invasion provide fast, easy and clinically useful recommendations that allow for the early prognostication of oral melanomas based on clinical size.

M3 - Article

JO - Journal of the American Veterinary Medical Association

JF - Journal of the American Veterinary Medical Association

SN - 0003-1488

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