Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes

Mengya Zhu, Ying Wang, Yi Deng, Li Yao, Samuel B. Adeloju, Daodong Pan, Feng Xue, Yucheng Wu, Lei Zheng, Wei Chen

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine-Hg2+-thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.
Original languageEnglish
Pages (from-to)14-20
Number of pages7
JournalBiosensors and Bioelectronics
Volume61
Early online date05 May 2014
DOIs
Publication statusPublished - 15 Nov 2014

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