Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes

Mengya Zhu; Ying Wang; Yi Deng; Li Yao; Samuel B. Adeloju; Daodong Pan; Feng Xue; Yucheng Wu; Lei Zheng; Wei Chen

doi:10.1016/j.bios.2014.04.049

Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes

Mengya Zhu, Ying Wang, Yi Deng, Li Yao, Samuel B. Adeloju, Daodong Pan, Feng Xue, Yucheng Wu, Lei Zheng, Wei Chen

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine-Hg2+-thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.

Original language	English
Pages (from-to)	14-20
Number of pages	7
Journal	Biosensors and Bioelectronics
Volume	61
Early online date	05 May 2014
DOIs	https://doi.org/10.1016/j.bios.2014.04.049
Publication status	Published - 15 Nov 2014

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Zhu, M., Wang, Y., Deng, Y., Yao, L., B. Adeloju, S., Pan, D., Xue, F., Wu, Y., Zheng, L., & Chen, W. (2014). Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes. Biosensors and Bioelectronics, 61, 14-20. Advance online publication. https://doi.org/10.1016/j.bios.2014.04.049

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title = "Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes",

abstract = "A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine-Hg2+-thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods. ",

keywords = "Food safety, Gold nanoparticles, Lateral flow strip, Signal amplification",

author = "Mengya Zhu and Ying Wang and Yi Deng and Li Yao and {B. Adeloju}, Samuel and Daodong Pan and Feng Xue and Yucheng Wu and Lei Zheng and Wei Chen",

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year = "2014",

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doi = "10.1016/j.bios.2014.04.049",

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AU - Zhu, Mengya

AU - Wang, Ying

AU - Deng, Yi

AU - Yao, Li

AU - B. Adeloju, Samuel

AU - Pan, Daodong

AU - Xue, Feng

AU - Wu, Yucheng

AU - Zheng, Lei

AU - Chen, Wei

N1 - Includes bibliographical references

PY - 2014/11/15

Y1 - 2014/11/15

N2 - A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine-Hg2+-thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.

AB - A novel one-step signal amplified lateral flow strip (SA-LFS) is described for direct ultrasensitive and on-site detection of Hg2+ based on the specific recognition system of thymine-Hg2+-thymine using gold nanoparticles (AuNPs) as labeling tags. The signal was also amplified by hybridization induced dual labeling of AuNPs, which increased signal intensity of test line on strips. The presence of both probes on the LFS enabled both sensing and signal amplification to be achieved in a single step. The resulting SA-LFS is capable of rapid and ultrasensitive detection of Hg2+ with LODs of 0.005ppb and 0.0015ppb by visual observation and quantitative analysis, respectively. At least 40-fold improvement in sensitivity of the traditional LFS was achieved through this signal amplification process. The successful application of the method to the determination of Hg2+ in water sample is reported. The proposed signal amplification process may also be applicable to other LFS-based methods.

KW - Food safety

KW - Gold nanoparticles

KW - Lateral flow strip

KW - Signal amplification

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Ultrasensitive detection of mercury with a novel one-step signal amplified lateral flow strip based on gold nanoparticle-labeled ssDNA recognition and enhancement probes

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