Ultrastructure of starches in two canned-bean products before and after digestion in vitro

Xiaohong Cao; Zhongkai Zhou; Jing Li; Fang Wang; Yumei Jiang

doi:10.1007/978-3-642-37916-1_62

Ultrastructure of starches in two canned-bean products before and after digestion in vitro

Xiaohong Cao, Zhongkai Zhou, Jing Li, Fang Wang, Yumei Jiang

Graham Centre for Agricultural Innovation

Research output: Book chapter/Published conference paper › Conference paper › peer-review

Abstract

The changes in physical and chemical structure of starches in bean products pre- and post-digestion in vitro were examined. In comparison with the morphology of starch in food, resistant starch (RS) in digesta exhibits a narrow size distribution with most particles being from 4 to 7 um. Fourier Transform Infrared Spectroscopy (FTIR) results showed that the absorbance band at 1022 cm-1, which represents amorphous/disordered starch in food, is sensitive to amylolysis, whereas the absorbance band at 1047 cm-1, which represents ordered starch was more pronounced in digesta. Scanning iodine spectrum results indicated that the average molecular weight of amylose in digesta was smaller than that in food. The molecular profile of RS showed a lack of higher molecular weight amylose. Study of SE-HPLC further proved that the amylopectin fraction is absent in RS molecular profile.

Original language	English
Title of host publication	Proceedings of the 2012 International Conference on Applied Biotechnology, ICAB 2012
Publisher	Springer-Verlag London Ltd.
Pages	609-618
Number of pages	10
Volume	249 LNEE
Edition	VOL. 1
ISBN (Print)	9783642379154
DOIs	https://doi.org/10.1007/978-3-642-37916-1_62
Publication status	Published - 01 Jun 2014
Event	2012 International Conference on Applied Biotechnology, ICAB 2012 - Tianjin, China Duration: 18 Oct 2012 → 19 Oct 2012

Publication series

Name	Lecture Notes in Electrical Engineering
Number	VOL. 1
Volume	249 LNEE
ISSN (Print)	1876-1100
ISSN (Electronic)	1876-1119

Conference

Conference	2012 International Conference on Applied Biotechnology, ICAB 2012
Country/Territory	China
City	Tianjin
Period	18/10/12 → 19/10/12
Other	The 2012 International Conference on Applied Biotechnology (ICAB 2012) was held in Tianjin, China on October 18-19, 2012. It provides not only a platform for domestic and foreign researchers to exchange their ideas and experiences with the application-oriented research of biotechnology, but also an opportunity to promote the development and prosperity of the biotechnology industry. The proceedings of ICAB 2012 mainly focus on the world's latest scientific research and techniques in applied biotechnology, including Industrial Microbial Technology, Food Biotechnology, Pharmaceutical Biotechnology, Environmental Biotechnology, Marine Biotechnology, Agricultural Biotechnology, Biological Materials and Bio-energy Technology, Advances in Biotechnology, and Future Trends in Biotechnology. These proceedings are intended for scientists and researchers engaging in applied biotechnology. Professor Pingkai Ouyang is the President of the Nanjing University of Technology, China. Professor Tongcun Zhang is the Director of the Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education at the College of Bioengineering, Tianjin University of Science and Technology, China. Dr. Samuel Kaplan is a Professor at the Department of Microbiology & Molecular Genetics at the University of Texas at Houston Medical School, Houston, Texas, USA. Dr. Bill Skarnes is a Professor at Wellcome Trust Sanger Institute, United Kingdom.

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10.1007/978-3-642-37916-1_62

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Cao, X., Zhou, Z., Li, J., Wang, F., & Jiang, Y. (2014). Ultrastructure of starches in two canned-bean products before and after digestion in vitro. In Proceedings of the 2012 International Conference on Applied Biotechnology, ICAB 2012 (VOL. 1 ed., Vol. 249 LNEE, pp. 609-618). (Lecture Notes in Electrical Engineering; Vol. 249 LNEE, No. VOL. 1). Springer-Verlag London Ltd.. https://doi.org/10.1007/978-3-642-37916-1_62

Cao, Xiaohong ; Zhou, Zhongkai ; Li, Jing ; Wang, Fang ; Jiang, Yumei. / Ultrastructure of starches in two canned-bean products before and after digestion in vitro. Proceedings of the 2012 International Conference on Applied Biotechnology, ICAB 2012. Vol. 249 LNEE VOL. 1. ed. Springer-Verlag London Ltd., 2014. pp. 609-618 (Lecture Notes in Electrical Engineering; VOL. 1).

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title = "Ultrastructure of starches in two canned-bean products before and after digestion in vitro",

abstract = "The changes in physical and chemical structure of starches in bean products pre- and post-digestion in vitro were examined. In comparison with the morphology of starch in food, resistant starch (RS) in digesta exhibits a narrow size distribution with most particles being from 4 to 7 um. Fourier Transform Infrared Spectroscopy (FTIR) results showed that the absorbance band at 1022 cm-1, which represents amorphous/disordered starch in food, is sensitive to amylolysis, whereas the absorbance band at 1047 cm-1, which represents ordered starch was more pronounced in digesta. Scanning iodine spectrum results indicated that the average molecular weight of amylose in digesta was smaller than that in food. The molecular profile of RS showed a lack of higher molecular weight amylose. Study of SE-HPLC further proved that the amylopectin fraction is absent in RS molecular profile.",

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author = "Xiaohong Cao and Zhongkai Zhou and Jing Li and Fang Wang and Yumei Jiang",

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Cao, X, Zhou, Z, Li, J, Wang, F & Jiang, Y 2014, Ultrastructure of starches in two canned-bean products before and after digestion in vitro. in Proceedings of the 2012 International Conference on Applied Biotechnology, ICAB 2012. VOL. 1 edn, vol. 249 LNEE, Lecture Notes in Electrical Engineering, no. VOL. 1, vol. 249 LNEE, Springer-Verlag London Ltd., pp. 609-618, 2012 International Conference on Applied Biotechnology, ICAB 2012, Tianjin, China, 18/10/12. https://doi.org/10.1007/978-3-642-37916-1_62

Ultrastructure of starches in two canned-bean products before and after digestion in vitro. / Cao, Xiaohong; Zhou, Zhongkai; Li, Jing; Wang, Fang; Jiang, Yumei.

Proceedings of the 2012 International Conference on Applied Biotechnology, ICAB 2012. Vol. 249 LNEE VOL. 1. ed. Springer-Verlag London Ltd., 2014. p. 609-618 (Lecture Notes in Electrical Engineering; Vol. 249 LNEE, No. VOL. 1).

Research output: Book chapter/Published conference paper › Conference paper › peer-review

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AU - Cao, Xiaohong

AU - Zhou, Zhongkai

AU - Li, Jing

AU - Wang, Fang

AU - Jiang, Yumei

PY - 2014/6/1

Y1 - 2014/6/1

N2 - The changes in physical and chemical structure of starches in bean products pre- and post-digestion in vitro were examined. In comparison with the morphology of starch in food, resistant starch (RS) in digesta exhibits a narrow size distribution with most particles being from 4 to 7 um. Fourier Transform Infrared Spectroscopy (FTIR) results showed that the absorbance band at 1022 cm-1, which represents amorphous/disordered starch in food, is sensitive to amylolysis, whereas the absorbance band at 1047 cm-1, which represents ordered starch was more pronounced in digesta. Scanning iodine spectrum results indicated that the average molecular weight of amylose in digesta was smaller than that in food. The molecular profile of RS showed a lack of higher molecular weight amylose. Study of SE-HPLC further proved that the amylopectin fraction is absent in RS molecular profile.

AB - The changes in physical and chemical structure of starches in bean products pre- and post-digestion in vitro were examined. In comparison with the morphology of starch in food, resistant starch (RS) in digesta exhibits a narrow size distribution with most particles being from 4 to 7 um. Fourier Transform Infrared Spectroscopy (FTIR) results showed that the absorbance band at 1022 cm-1, which represents amorphous/disordered starch in food, is sensitive to amylolysis, whereas the absorbance band at 1047 cm-1, which represents ordered starch was more pronounced in digesta. Scanning iodine spectrum results indicated that the average molecular weight of amylose in digesta was smaller than that in food. The molecular profile of RS showed a lack of higher molecular weight amylose. Study of SE-HPLC further proved that the amylopectin fraction is absent in RS molecular profile.

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Cao X, Zhou Z, Li J, Wang F, Jiang Y. Ultrastructure of starches in two canned-bean products before and after digestion in vitro. In Proceedings of the 2012 International Conference on Applied Biotechnology, ICAB 2012. VOL. 1 ed. Vol. 249 LNEE. Springer-Verlag London Ltd. 2014. p. 609-618. (Lecture Notes in Electrical Engineering; VOL. 1). https://doi.org/10.1007/978-3-642-37916-1_62

Ultrastructure of starches in two canned-bean products before and after digestion in vitro

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