Ultrastructure of starches in two canned-bean products before and after digestion in vitro

Xiaohong Cao, Zhongkai Zhou, Jing Li, Fang Wang, Yumei Jiang

    Research output: Book chapter/Published conference paperConference paperpeer-review


    The changes in physical and chemical structure of starches in bean products pre- and post-digestion in vitro were examined. In comparison with the morphology of starch in food, resistant starch (RS) in digesta exhibits a narrow size distribution with most particles being from 4 to 7 um. Fourier Transform Infrared Spectroscopy (FTIR) results showed that the absorbance band at 1022 cm-1, which represents amorphous/disordered starch in food, is sensitive to amylolysis, whereas the absorbance band at 1047 cm-1, which represents ordered starch was more pronounced in digesta. Scanning iodine spectrum results indicated that the average molecular weight of amylose in digesta was smaller than that in food. The molecular profile of RS showed a lack of higher molecular weight amylose. Study of SE-HPLC further proved that the amylopectin fraction is absent in RS molecular profile.
    Original languageEnglish
    Title of host publicationProceedings of the 2012 International Conference on Applied Biotechnology, ICAB 2012
    PublisherSpringer-Verlag London Ltd.
    Number of pages10
    Volume249 LNEE
    EditionVOL. 1
    ISBN (Print)9783642379154
    Publication statusPublished - 01 Jun 2014
    Event2012 International Conference on Applied Biotechnology, ICAB 2012 - Tianjin, China
    Duration: 18 Oct 201219 Oct 2012

    Publication series

    NameLecture Notes in Electrical Engineering
    NumberVOL. 1
    Volume249 LNEE
    ISSN (Print)1876-1100
    ISSN (Electronic)1876-1119


    Conference2012 International Conference on Applied Biotechnology, ICAB 2012
    OtherThe 2012 International Conference on Applied Biotechnology (ICAB 2012) was held in Tianjin, China on October 18-19, 2012. It provides not only a platform for domestic and foreign researchers to exchange their ideas and experiences with the application-oriented research of biotechnology, but also an opportunity to promote the development and prosperity of the biotechnology industry. The proceedings of ICAB 2012 mainly focus on the world's latest scientific research and techniques in applied biotechnology, including Industrial Microbial Technology, Food Biotechnology, Pharmaceutical Biotechnology, Environmental Biotechnology, Marine Biotechnology, Agricultural Biotechnology, Biological Materials and Bio-energy Technology, Advances in Biotechnology, and Future Trends in Biotechnology. These proceedings are intended for scientists and researchers engaging in applied biotechnology. Professor Pingkai Ouyang is the President of the Nanjing University of Technology, China. Professor Tongcun Zhang is the Director of the Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education at the College of Bioengineering, Tianjin University of Science and Technology, China. Dr. Samuel Kaplan is a Professor at the Department of Microbiology & Molecular Genetics at the University of Texas at Houston Medical School, Houston, Texas, USA. Dr. Bill Skarnes is a Professor at Wellcome Trust Sanger Institute, United Kingdom.


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