Use of matrix-assisted laser desorption ionization–time of flight mass spectrometry to identify MLST clade 4 Clostridium difficile isolates

Jing Wei Cheng, Chang Liu, Timothy Kudinha, Meng Xiao, Shu Ying Yu, Chun Xia Yang, Ming Wei, Guo Wei Liang, Dong Hua Shao, Fanrong Kong, Zhao Hui Tong, Ying Chun Xu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Clostridium difficile is the leading cause of health care-associated infections. Previous studies suggest that C. difficile MLST clade 4 strains with higher drug resistance rates constitute the major clone spreading in China. Thus development of a rapid and accurate typing method for these strains is needed to monitor the epidemiology of this clone and to guide clinical treatment. A total of 160 non-duplicate C. difficile isolates recovered from three large teaching hospitals in Beijing were studied. All the 41 clade 4 C. difficile isolates clustered together on the PCA dendrogram. Spectra peak statistics revealed that five markers (2691.43Da, 2704.91Da, 2711.93Da, 3247.27Da and 3290.76Da) can easily and reliably distinguish between clade 4 and non-clade 4 isolates, with area under the curve (AUC) values of 0.991, 0.997, 0.973, 1 and 1, respectively. In conclusion, MALDI-TOF MS is a very simple and accurate method for identifying C. difficile MLST clade 4 strains.
Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume92
Issue number1
DOIs
Publication statusPublished - Apr 2018

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