Vaccine Development for Prescottella Equi

Thirumahal Vanniasinkam, C Giles, S Ndi, M Heuzenroeder, M Barton

Research output: Book chapter/Published conference paperConference paper

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Abstract

Prescottella equi (formerly Rhodococcus equi) is an intracellular pathogen that causes pyogranulomatous pneumonia in Thoroughbred foals. There is currently no vaccine available for the prevention of this disease in foals despite years of research. Cell mediated immunity is considered crucial for overcoming an infection caused by this pathogen. The virulence associated protein (VapA) is a well characterized immunogenic protein associated with this pathogen and was used to develop DNA and recombinant protein vaccines in this study. Vaccine candidates and live P. equi based vaccine were tested in BALB/c mice. Mice were challenged with virulent P.equi 2 weeks following the last boost and IgG subtypes and bacterial clearance from spleen and liver determined. The DNA vaccine elicited a significant IgG2a response indicating a Th1 biased immune response. The IFN gamma response from DNA and recombinant VapA vaccinated mice was moderate. The results of the challenge study showed that neither the recombinant VapA protein nor DNA vaccine enhanced clearance of P. equi in this model.
Original languageEnglish
Title of host publicationProcedia in Vaccinology
Subtitle of host publication7th Vaccine & ISV Annual Global Congress
EditorsRay Spier
Place of PublicationNetherlands
PublisherElsevier
Pages50-57
Number of pages8
Volume8
DOIs
Publication statusPublished - 2014
Event7th Vaccine & ISV Congress - Meliá Sitges, Sitges, Barcelona, Spain
Duration: 27 Oct 201329 Oct 2013
https://www.isv-online.org/menu-annual-congress/previos-congress/7th-global-congress-oral-programme

Publication series

Name
ISSN (Print)1877-282X

Conference

Conference7th Vaccine & ISV Congress
CountrySpain
CitySitges, Barcelona
Period27/10/1329/10/13
Internet address

Fingerprint

DNA Vaccines
Vaccines
Recombinant Proteins
Rhodococcus equi
Recombinant DNA
Cellular Immunity
Virulence
Pneumonia
Proteins
Spleen
Immunoglobulin G
Liver
Infection
Research

Cite this

Vanniasinkam, T., Giles, C., Ndi, S., Heuzenroeder, M., & Barton, M. (2014). Vaccine Development for Prescottella Equi. In R. Spier (Ed.), Procedia in Vaccinology: 7th Vaccine & ISV Annual Global Congress (Vol. 8, pp. 50-57). Netherlands: Elsevier. https://doi.org/10.1016/j.provac.2014.07.009
Vanniasinkam, Thirumahal ; Giles, C ; Ndi, S ; Heuzenroeder, M ; Barton, M. / Vaccine Development for Prescottella Equi. Procedia in Vaccinology: 7th Vaccine & ISV Annual Global Congress. editor / Ray Spier. Vol. 8 Netherlands : Elsevier, 2014. pp. 50-57
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abstract = "Prescottella equi (formerly Rhodococcus equi) is an intracellular pathogen that causes pyogranulomatous pneumonia in Thoroughbred foals. There is currently no vaccine available for the prevention of this disease in foals despite years of research. Cell mediated immunity is considered crucial for overcoming an infection caused by this pathogen. The virulence associated protein (VapA) is a well characterized immunogenic protein associated with this pathogen and was used to develop DNA and recombinant protein vaccines in this study. Vaccine candidates and live P. equi based vaccine were tested in BALB/c mice. Mice were challenged with virulent P.equi 2 weeks following the last boost and IgG subtypes and bacterial clearance from spleen and liver determined. The DNA vaccine elicited a significant IgG2a response indicating a Th1 biased immune response. The IFN gamma response from DNA and recombinant VapA vaccinated mice was moderate. The results of the challenge study showed that neither the recombinant VapA protein nor DNA vaccine enhanced clearance of P. equi in this model.",
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Vanniasinkam, T, Giles, C, Ndi, S, Heuzenroeder, M & Barton, M 2014, Vaccine Development for Prescottella Equi. in R Spier (ed.), Procedia in Vaccinology: 7th Vaccine & ISV Annual Global Congress. vol. 8, Elsevier, Netherlands, pp. 50-57, 7th Vaccine & ISV Congress, Sitges, Barcelona, Spain, 27/10/13. https://doi.org/10.1016/j.provac.2014.07.009

Vaccine Development for Prescottella Equi. / Vanniasinkam, Thirumahal; Giles, C; Ndi, S; Heuzenroeder, M; Barton, M.

Procedia in Vaccinology: 7th Vaccine & ISV Annual Global Congress. ed. / Ray Spier. Vol. 8 Netherlands : Elsevier, 2014. p. 50-57.

Research output: Book chapter/Published conference paperConference paper

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AU - Heuzenroeder, M

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N1 - Imported on 16 May 2017 - DigiTool details were: publisher = Netherlands: Elsevier, 2014. editor/s (773b) = Ray Spier; Event dates (773o) = 27 -29 October 2013; Volume no. (773r) = 8; Parent title (773t) = Vaccine & ISV Congress. ISSNs: 1877-282X;

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N2 - Prescottella equi (formerly Rhodococcus equi) is an intracellular pathogen that causes pyogranulomatous pneumonia in Thoroughbred foals. There is currently no vaccine available for the prevention of this disease in foals despite years of research. Cell mediated immunity is considered crucial for overcoming an infection caused by this pathogen. The virulence associated protein (VapA) is a well characterized immunogenic protein associated with this pathogen and was used to develop DNA and recombinant protein vaccines in this study. Vaccine candidates and live P. equi based vaccine were tested in BALB/c mice. Mice were challenged with virulent P.equi 2 weeks following the last boost and IgG subtypes and bacterial clearance from spleen and liver determined. The DNA vaccine elicited a significant IgG2a response indicating a Th1 biased immune response. The IFN gamma response from DNA and recombinant VapA vaccinated mice was moderate. The results of the challenge study showed that neither the recombinant VapA protein nor DNA vaccine enhanced clearance of P. equi in this model.

AB - Prescottella equi (formerly Rhodococcus equi) is an intracellular pathogen that causes pyogranulomatous pneumonia in Thoroughbred foals. There is currently no vaccine available for the prevention of this disease in foals despite years of research. Cell mediated immunity is considered crucial for overcoming an infection caused by this pathogen. The virulence associated protein (VapA) is a well characterized immunogenic protein associated with this pathogen and was used to develop DNA and recombinant protein vaccines in this study. Vaccine candidates and live P. equi based vaccine were tested in BALB/c mice. Mice were challenged with virulent P.equi 2 weeks following the last boost and IgG subtypes and bacterial clearance from spleen and liver determined. The DNA vaccine elicited a significant IgG2a response indicating a Th1 biased immune response. The IFN gamma response from DNA and recombinant VapA vaccinated mice was moderate. The results of the challenge study showed that neither the recombinant VapA protein nor DNA vaccine enhanced clearance of P. equi in this model.

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Vanniasinkam T, Giles C, Ndi S, Heuzenroeder M, Barton M. Vaccine Development for Prescottella Equi. In Spier R, editor, Procedia in Vaccinology: 7th Vaccine & ISV Annual Global Congress. Vol. 8. Netherlands: Elsevier. 2014. p. 50-57 https://doi.org/10.1016/j.provac.2014.07.009