Vascular function in berries of Vitis vinifera (L) cv Shiraz

Suzy Rogiers, Jason Smith, Rosemary White, Markus Keller, Bruno Holzapfel, James Virgona

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Anatomical studies on the movement of a xylem tracer dye were combined with functional studies on changes in grape berry volume during final stages of berry ripening to gauge xylem effectiveness. Movement of a xylem tracer dye into pre-veraison fruit was compared with movement into post-veraison fruit by feeding a solution of acid fuchsin to excised shoots with bunches still attached, and then sectioning fruit for photo-microscopy. Those comparisons confirmed published studies showing an apparent blockage to dye movement along major vessels within the brush tissue of post-veraison fruit. However, our functional approach yielded a different impression of vascular activity. A continuation of xylem transport in ripening fruit was inferred from comparisons of berry volume where pedicels were either girdled (phloem interrupted, but xylem intact) or excised (both phloem and xylem interrupted). Volume changes in manipulated berries were compared with immediately adjacent intact control berries within the same bunch. Control fruit lost volume subsequent to 78 days after flowering (DAF) while manipulated fruit lost volume from the first day of treatment at 67 DAF. By harvest time at 95 DAF, both control fruit and girdled fruit had fallen to 91% and excised fruit to 46% of maximum volumes recorded around 78 DAF. Berry volume loss in girdled fruit was further enhanced by deficit irrigation. We conclude that xylem flow into those Shiraz berries must have continued beyond veraison despite dye evidence of a vessel blockage within the brush region of analogous post-veraison fruit.
Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalAustralian Journal of Grape and Wine Research
Volume7
Issue number1
DOIs
Publication statusPublished - 2001

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blood vessels
Vitis vinifera
small fruits
fruits
xylem
dyes
flowering
xylem vessels
phloem
tracer techniques
ripening
deficit irrigation
pedicel
gauges
grapes
microscopy
shoots

Cite this

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title = "Vascular function in berries of Vitis vinifera (L) cv Shiraz",
abstract = "Anatomical studies on the movement of a xylem tracer dye were combined with functional studies on changes in grape berry volume during final stages of berry ripening to gauge xylem effectiveness. Movement of a xylem tracer dye into pre-veraison fruit was compared with movement into post-veraison fruit by feeding a solution of acid fuchsin to excised shoots with bunches still attached, and then sectioning fruit for photo-microscopy. Those comparisons confirmed published studies showing an apparent blockage to dye movement along major vessels within the brush tissue of post-veraison fruit. However, our functional approach yielded a different impression of vascular activity. A continuation of xylem transport in ripening fruit was inferred from comparisons of berry volume where pedicels were either girdled (phloem interrupted, but xylem intact) or excised (both phloem and xylem interrupted). Volume changes in manipulated berries were compared with immediately adjacent intact control berries within the same bunch. Control fruit lost volume subsequent to 78 days after flowering (DAF) while manipulated fruit lost volume from the first day of treatment at 67 DAF. By harvest time at 95 DAF, both control fruit and girdled fruit had fallen to 91{\%} and excised fruit to 46{\%} of maximum volumes recorded around 78 DAF. Berry volume loss in girdled fruit was further enhanced by deficit irrigation. We conclude that xylem flow into those Shiraz berries must have continued beyond veraison despite dye evidence of a vessel blockage within the brush region of analogous post-veraison fruit.",
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Vascular function in berries of Vitis vinifera (L) cv Shiraz. / Rogiers, Suzy; Smith, Jason; White, Rosemary; Keller, Markus; Holzapfel, Bruno; Virgona, James.

In: Australian Journal of Grape and Wine Research, Vol. 7, No. 1, 2001, p. 46-51.

Research output: Contribution to journalArticle

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T1 - Vascular function in berries of Vitis vinifera (L) cv Shiraz

AU - Rogiers, Suzy

AU - Smith, Jason

AU - White, Rosemary

AU - Keller, Markus

AU - Holzapfel, Bruno

AU - Virgona, James

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Australian Journal of Grape and Wine Research. ISSNs: 1322-7130;

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SN - 1322-7130

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ER -