Vascular patterns in grape

Zeyu Xiao, Suzy Rogiers, Leigh Schmidtke, Steve Tyerman, Rosemary White, Aude Gourieroux, Sabrina Chin, Vinay Pagay

Research output: Other contribution to conferencePoster

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Abstract

Vascular bundles are the main transport channels of water and nutrients into grape berries, and play a very important role in fruit development and quality. During the late ripening stages, water flow through the xylem declines significantly. Phloem transport takes over in supplying water for further growth and water replacement to transpirational loss. Xylem may serve as the channel for water backflow and recycling of excess phloem water. The balance of water dynamics dictates yield and fruit quality. How the vascular bundles connect from the pedicel into the grape berry at brush zone remains unclear. We used fluorescent microscopy to document the morphology and anatomy of brush zone vascular bundles of four grape cultivars. Fresh and methanol stored berries of Shiraz (BVRC 12 & 1654), Sauvignon Blanc, Ruby Seedless and Flame Seedless were hand sectioned and stained with Aniline Blue Fluorochrome and Acridine Orange respectively. A distinctive change in vascular arrangement from the pedicel into the grape brush zone was apparent in all examined cultivars. At the junction of the receptacle (stem) and the berry pericarp, the vascular bundles exhibited the arrangement where xylem was surrounded by phloem. In the brush zone, the vascular bundles reoriented so that the phloem was situated on the inner side of the vascular bundles. This is the first study to characterise the vascular patterns of the grape berry brush zone of four commercial cultivars.
Original languageEnglish
Publication statusPublished - 2019
Event17th Australian Wine Industry Technical Conference (AWITC 2019) - Adelaide Convention Centre, Adelaide, Australia
Duration: 21 Jul 201924 Jul 2019
https://awitc.com.au

Conference

Conference17th Australian Wine Industry Technical Conference (AWITC 2019)
CountryAustralia
CityAdelaide
Period21/07/1924/07/19
Internet address

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vascular bundles
blood vessels
grapes
phloem
xylem
pedicel
water
small fruits
fruit quality
cultivars
acridine orange
aniline
fluorescent dyes
pericarp
water balance
water flow
recycling
fruiting
microscopy
ripening

Cite this

Xiao, Z., Rogiers, S., Schmidtke, L., Tyerman, S., White, R., Gourieroux, A., ... Pagay, V. (2019). Vascular patterns in grape. Poster session presented at 17th Australian Wine Industry Technical Conference (AWITC 2019), Adelaide, Australia.
Xiao, Zeyu ; Rogiers, Suzy ; Schmidtke, Leigh ; Tyerman, Steve ; White, Rosemary ; Gourieroux, Aude ; Chin, Sabrina ; Pagay, Vinay. / Vascular patterns in grape. Poster session presented at 17th Australian Wine Industry Technical Conference (AWITC 2019), Adelaide, Australia.
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Xiao, Z, Rogiers, S, Schmidtke, L, Tyerman, S, White, R, Gourieroux, A, Chin, S & Pagay, V 2019, 'Vascular patterns in grape', 17th Australian Wine Industry Technical Conference (AWITC 2019), Adelaide, Australia, 21/07/19 - 24/07/19.

Vascular patterns in grape. / Xiao, Zeyu; Rogiers, Suzy; Schmidtke, Leigh; Tyerman, Steve; White, Rosemary; Gourieroux, Aude; Chin, Sabrina; Pagay, Vinay.

2019. Poster session presented at 17th Australian Wine Industry Technical Conference (AWITC 2019), Adelaide, Australia.

Research output: Other contribution to conferencePoster

TY - CONF

T1 - Vascular patterns in grape

AU - Xiao, Zeyu

AU - Rogiers, Suzy

AU - Schmidtke, Leigh

AU - Tyerman, Steve

AU - White, Rosemary

AU - Gourieroux, Aude

AU - Chin, Sabrina

AU - Pagay, Vinay

PY - 2019

Y1 - 2019

N2 - Vascular bundles are the main transport channels of water and nutrients into grape berries, and play a very important role in fruit development and quality. During the late ripening stages, water flow through the xylem declines significantly. Phloem transport takes over in supplying water for further growth and water replacement to transpirational loss. Xylem may serve as the channel for water backflow and recycling of excess phloem water. The balance of water dynamics dictates yield and fruit quality. How the vascular bundles connect from the pedicel into the grape berry at brush zone remains unclear. We used fluorescent microscopy to document the morphology and anatomy of brush zone vascular bundles of four grape cultivars. Fresh and methanol stored berries of Shiraz (BVRC 12 & 1654), Sauvignon Blanc, Ruby Seedless and Flame Seedless were hand sectioned and stained with Aniline Blue Fluorochrome and Acridine Orange respectively. A distinctive change in vascular arrangement from the pedicel into the grape brush zone was apparent in all examined cultivars. At the junction of the receptacle (stem) and the berry pericarp, the vascular bundles exhibited the arrangement where xylem was surrounded by phloem. In the brush zone, the vascular bundles reoriented so that the phloem was situated on the inner side of the vascular bundles. This is the first study to characterise the vascular patterns of the grape berry brush zone of four commercial cultivars.

AB - Vascular bundles are the main transport channels of water and nutrients into grape berries, and play a very important role in fruit development and quality. During the late ripening stages, water flow through the xylem declines significantly. Phloem transport takes over in supplying water for further growth and water replacement to transpirational loss. Xylem may serve as the channel for water backflow and recycling of excess phloem water. The balance of water dynamics dictates yield and fruit quality. How the vascular bundles connect from the pedicel into the grape berry at brush zone remains unclear. We used fluorescent microscopy to document the morphology and anatomy of brush zone vascular bundles of four grape cultivars. Fresh and methanol stored berries of Shiraz (BVRC 12 & 1654), Sauvignon Blanc, Ruby Seedless and Flame Seedless were hand sectioned and stained with Aniline Blue Fluorochrome and Acridine Orange respectively. A distinctive change in vascular arrangement from the pedicel into the grape brush zone was apparent in all examined cultivars. At the junction of the receptacle (stem) and the berry pericarp, the vascular bundles exhibited the arrangement where xylem was surrounded by phloem. In the brush zone, the vascular bundles reoriented so that the phloem was situated on the inner side of the vascular bundles. This is the first study to characterise the vascular patterns of the grape berry brush zone of four commercial cultivars.

KW - Vascular

KW - grape berries

M3 - Poster

ER -

Xiao Z, Rogiers S, Schmidtke L, Tyerman S, White R, Gourieroux A et al. Vascular patterns in grape. 2019. Poster session presented at 17th Australian Wine Industry Technical Conference (AWITC 2019), Adelaide, Australia.