Viral load, shedding rate and lateral transmission of Marek's disease vaccinal virus (Rispens/CVI988) in SPF chickens.

T. Islam, Katrin Renz, S.W. Walkden-Brown

Research output: Book chapter/Published conference paperConference paperpeer-review

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Abstract

The Rispens (CVI988) attenuated Serotype 1 Marek's Disease virus vaccine is highly effective and used worldwide. It has been shown that this virus is present in feather tips of vaccinated chickens but the extent to which current commercial Rispens vaccines transmit effectively between chickens is unknown. To determine this, we measured the shedding rate, lateral transmission and changes in viral load over time, of the three commercial Rispens vaccines available in Australia. In each of three climate-controlled rooms, 10 SPF (specific pathogen free) chickens were vaccinated with a commercial Rispens vaccine at day old and left in contact with 10 unvaccinated chickens. A separate room contained unvaccinated control birds. As determined by quantitative real-time polymerase chain reaction (PCR) of room dust and peripheral blood lymphocytes (PBL), vaccine virus was shed from the vaccinated chickens in dander from day 7 and transmitted effectively from vaccinated to in-contact chickens with a lag period of 2-3 weeks.
Original languageEnglish
Title of host publication22nd Annual Proceedings
Place of PublicationSydney, Australia
PublisherFaculty of Veterinary Science, University of Sydney
Pages239-242
Number of pages4
Publication statusPublished - 2011
EventAustralian Poultry Science Symposium - Sydney, NSW, Australia
Duration: 14 Feb 201116 Feb 2011

Conference

ConferenceAustralian Poultry Science Symposium
Country/TerritoryAustralia
Period14/02/1116/02/11

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