Volatile organic compounds produced by Aureobasidium pullulans inhibit the growth of Botrytis cinerea and Alternaria alternata

Research output: Other contribution to conferenceAbstract

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Abstract

Botrytis cinerea and Alternaria alternata are two necrotrophic fungal pathogens causing grey and black mould respectively on horticultural crops, including tomatoes and grapes, leading to enormous preharvest and postharvest losses worldwide. Aureobasidium pullulans is a yeast-like saprophytic fungus which naturally inhabits plant and fruit surfaces and is a potential biocontrol agent against a wide range of pathogenic fungi. Production of antifungal volatile organic compounds (VOC) has been postulated as one of the biocontrol mechanisms of A. pullulans. In this study the efficacy of antifungal VOC from four A. pullulans isolates were tested in vitro against two B. cinerea isolates from tomato and wine grapes and one A. alternata isolate from tomato using a double Petri dish assay. Two base Petri dishes containing PDA were stacked open-end to open-end. A. pullulans was inoculated on the upper dish and the pathogen inoculated on the second (lower). Inhibition of radial growth of the pathogen colony was measured. In a second experiment a Petri dish containing a glass slide with a pathogen conidial suspension in PDB was exposed to the A. pullulans inoculated dish to assess the inhibition of conidia germination. Exposure to the headspace of four A. pullulans isolates caused a 37- 85 % reduction of colony diameter of B. cinerea and 35-47 % for A. alternata compared to non-fumigated controls after three days of incubation at 25 °C. A. pullulans VOC also inhibited conidial germination of the three pathogen isolates, by 29-75% and 22-83% for the A. alternata and B. cinerea isolates respectively. Morphological deformations were observed in fumigated conidia and germ tubes of the pathogens when exposed to the headspace of the A. pullulans culture. To elucidate the nature of the A. pullulans VOC, the culture headspace was analysed by SPME-GC-MS. Thirteen VOC were identified that included alcohols, ketones and esters
Original languageEnglish
Publication statusPublished - 2019
EventV International Symposium on Postharvest Pathology - University of Liege, Liege, Belgium
Duration: 19 May 201924 May 2019

Conference

ConferenceV International Symposium on Postharvest Pathology
CountryBelgium
CityLiege
Period19/05/1924/05/19

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Aureobasidium pullulans
Alternaria alternata
volatile organic compounds
Botrytis cinerea
pathogens
headspace analysis
tomatoes
conidia
germination
postharvest losses
wine grapes
fungi
germ tube
horticultural crops
ketones
molds (fungi)
biological control agents
glass
grapes
biological control

Cite this

@conference{d0f7c83a7af34f609f9b05dc45958fbb,
title = "Volatile organic compounds produced by Aureobasidium pullulans inhibit the growth of Botrytis cinerea and Alternaria alternata",
abstract = "Botrytis cinerea and Alternaria alternata are two necrotrophic fungal pathogens causing grey and black mould respectively on horticultural crops, including tomatoes and grapes, leading to enormous preharvest and postharvest losses worldwide. Aureobasidium pullulans is a yeast-like saprophytic fungus which naturally inhabits plant and fruit surfaces and is a potential biocontrol agent against a wide range of pathogenic fungi. Production of antifungal volatile organic compounds (VOC) has been postulated as one of the biocontrol mechanisms of A. pullulans. In this study the efficacy of antifungal VOC from four A. pullulans isolates were tested in vitro against two B. cinerea isolates from tomato and wine grapes and one A. alternata isolate from tomato using a double Petri dish assay. Two base Petri dishes containing PDA were stacked open-end to open-end. A. pullulans was inoculated on the upper dish and the pathogen inoculated on the second (lower). Inhibition of radial growth of the pathogen colony was measured. In a second experiment a Petri dish containing a glass slide with a pathogen conidial suspension in PDB was exposed to the A. pullulans inoculated dish to assess the inhibition of conidia germination. Exposure to the headspace of four A. pullulans isolates caused a 37- 85 {\%} reduction of colony diameter of B. cinerea and 35-47 {\%} for A. alternata compared to non-fumigated controls after three days of incubation at 25 °C. A. pullulans VOC also inhibited conidial germination of the three pathogen isolates, by 29-75{\%} and 22-83{\%} for the A. alternata and B. cinerea isolates respectively. Morphological deformations were observed in fumigated conidia and germ tubes of the pathogens when exposed to the headspace of the A. pullulans culture. To elucidate the nature of the A. pullulans VOC, the culture headspace was analysed by SPME-GC-MS. Thirteen VOC were identified that included alcohols, ketones and esters",
author = "{Yalage Don}, Sashika and Leigh Schmidtke and Joanna Gambetta and Christopher Steel",
year = "2019",
language = "English",
note = "V International Symposium on Postharvest Pathology ; Conference date: 19-05-2019 Through 24-05-2019",

}

Volatile organic compounds produced by Aureobasidium pullulans inhibit the growth of Botrytis cinerea and Alternaria alternata. / Yalage Don, Sashika; Schmidtke, Leigh; Gambetta, Joanna; Steel, Christopher.

2019. Abstract from V International Symposium on Postharvest Pathology, Liege, Belgium.

Research output: Other contribution to conferenceAbstract

TY - CONF

T1 - Volatile organic compounds produced by Aureobasidium pullulans inhibit the growth of Botrytis cinerea and Alternaria alternata

AU - Yalage Don, Sashika

AU - Schmidtke, Leigh

AU - Gambetta, Joanna

AU - Steel, Christopher

PY - 2019

Y1 - 2019

N2 - Botrytis cinerea and Alternaria alternata are two necrotrophic fungal pathogens causing grey and black mould respectively on horticultural crops, including tomatoes and grapes, leading to enormous preharvest and postharvest losses worldwide. Aureobasidium pullulans is a yeast-like saprophytic fungus which naturally inhabits plant and fruit surfaces and is a potential biocontrol agent against a wide range of pathogenic fungi. Production of antifungal volatile organic compounds (VOC) has been postulated as one of the biocontrol mechanisms of A. pullulans. In this study the efficacy of antifungal VOC from four A. pullulans isolates were tested in vitro against two B. cinerea isolates from tomato and wine grapes and one A. alternata isolate from tomato using a double Petri dish assay. Two base Petri dishes containing PDA were stacked open-end to open-end. A. pullulans was inoculated on the upper dish and the pathogen inoculated on the second (lower). Inhibition of radial growth of the pathogen colony was measured. In a second experiment a Petri dish containing a glass slide with a pathogen conidial suspension in PDB was exposed to the A. pullulans inoculated dish to assess the inhibition of conidia germination. Exposure to the headspace of four A. pullulans isolates caused a 37- 85 % reduction of colony diameter of B. cinerea and 35-47 % for A. alternata compared to non-fumigated controls after three days of incubation at 25 °C. A. pullulans VOC also inhibited conidial germination of the three pathogen isolates, by 29-75% and 22-83% for the A. alternata and B. cinerea isolates respectively. Morphological deformations were observed in fumigated conidia and germ tubes of the pathogens when exposed to the headspace of the A. pullulans culture. To elucidate the nature of the A. pullulans VOC, the culture headspace was analysed by SPME-GC-MS. Thirteen VOC were identified that included alcohols, ketones and esters

AB - Botrytis cinerea and Alternaria alternata are two necrotrophic fungal pathogens causing grey and black mould respectively on horticultural crops, including tomatoes and grapes, leading to enormous preharvest and postharvest losses worldwide. Aureobasidium pullulans is a yeast-like saprophytic fungus which naturally inhabits plant and fruit surfaces and is a potential biocontrol agent against a wide range of pathogenic fungi. Production of antifungal volatile organic compounds (VOC) has been postulated as one of the biocontrol mechanisms of A. pullulans. In this study the efficacy of antifungal VOC from four A. pullulans isolates were tested in vitro against two B. cinerea isolates from tomato and wine grapes and one A. alternata isolate from tomato using a double Petri dish assay. Two base Petri dishes containing PDA were stacked open-end to open-end. A. pullulans was inoculated on the upper dish and the pathogen inoculated on the second (lower). Inhibition of radial growth of the pathogen colony was measured. In a second experiment a Petri dish containing a glass slide with a pathogen conidial suspension in PDB was exposed to the A. pullulans inoculated dish to assess the inhibition of conidia germination. Exposure to the headspace of four A. pullulans isolates caused a 37- 85 % reduction of colony diameter of B. cinerea and 35-47 % for A. alternata compared to non-fumigated controls after three days of incubation at 25 °C. A. pullulans VOC also inhibited conidial germination of the three pathogen isolates, by 29-75% and 22-83% for the A. alternata and B. cinerea isolates respectively. Morphological deformations were observed in fumigated conidia and germ tubes of the pathogens when exposed to the headspace of the A. pullulans culture. To elucidate the nature of the A. pullulans VOC, the culture headspace was analysed by SPME-GC-MS. Thirteen VOC were identified that included alcohols, ketones and esters

M3 - Abstract

ER -