West Nile virus genome with glycosylated envelope protein and deletion of alpha helices 1, 2, and 4 in the capsid protein is noninfectious and efficiently secretes subviral particles

Justin A Roby, Roy A Hall, Alexander A Khromykh

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Flavivirus genomes with deletions in the capsid (C) gene are attractive vaccine candidates, as they secrete highly immunogenic subviral particles (SVPs) without generating infectious virus. Here, we report that cytomegalovirus promoter-driven cDNA of West Nile virus Kunjin (KUNV) containing a glycosylation motif in the envelope (E) gene and a combined deletion of alpha helices 1, 2, and 4 in C produces significantly more SVPs than KUNV cDNAs with nonglycosylated E and various other deletions in C.

Original languageEnglish
Pages (from-to)13063-9
Number of pages7
JournalJournal of Virology
Volume87
Issue number23
DOIs
Publication statusPublished - Dec 2013

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