White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats

Christina M. Davy, Michael E. Donaldson, Sonu Subudhi, Noreen Rapin, Lisa Warnecke, James M. Turner, Trent K. Bollinger, Christopher J. Kyle, Nicole A.S.Y. Dorville, Emma L. Kunkel, Kaleigh J.O. Norquay, Yvonne A. Dzal, Craig K.R. Willis, Vikram Misra

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Abstract

Spillover of viruses from bats to other animals may be associated with increased contact between them, as well as increased shedding of viruses by bats. Here, we tested the prediction that little brown bats (Myotis lucifugus) co-infected with the M. lucifugus coronavirus (Myl-CoV) and with Pseudogymnoascus destructans (Pd), the fungus that causes bat white-nose syndrome (WNS), exhibit different disease severity, viral shedding and molecular responses than bats infected with only Myl-CoV or only P. destructans. We took advantage of the natural persistence of Myl-CoV in bats that were experimentally inoculated with P. destructans in a previous study. Here, we show that the intestines of virus-infected bats that were also infected with fungus contained on average 60-fold more viral RNA than bats with virus alone. Increased viral RNA in the intestines correlated with the severity of fungus-related pathology. Additionally, the intestines of bats infected with fungus exhibited different expression of mitogen-activated protein kinase pathway and cytokine related transcripts, irrespective of viral presence. Levels of coronavirus antibodies were also higher in fungal-infected bats. Our results suggest that the systemic effects of WNS may down-regulate anti-viral responses in bats persistently infected with M. lucifugus coronavirus and increase the potential of virus shedding.
Original languageEnglish
Article number15508
Pages (from-to)1-12
Number of pages12
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 19 Oct 2018

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Coronavirus
Nose
Virus Shedding
Fungi
Intestines
Viral RNA
Viruses
Progressive Myoclonic Epilepsy
Mitogen-Activated Protein Kinases
Down-Regulation
Pathology
Cytokines

Cite this

Davy, Christina M. ; Donaldson, Michael E. ; Subudhi, Sonu ; Rapin, Noreen ; Warnecke, Lisa ; Turner, James M. ; Bollinger, Trent K. ; Kyle, Christopher J. ; Dorville, Nicole A.S.Y. ; Kunkel, Emma L. ; Norquay, Kaleigh J.O. ; Dzal, Yvonne A. ; Willis, Craig K.R. ; Misra, Vikram. / White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats. In: Scientific Reports. 2018 ; Vol. 8, No. 1. pp. 1-12.
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abstract = "Spillover of viruses from bats to other animals may be associated with increased contact between them, as well as increased shedding of viruses by bats. Here, we tested the prediction that little brown bats (Myotis lucifugus) co-infected with the M. lucifugus coronavirus (Myl-CoV) and with Pseudogymnoascus destructans (Pd), the fungus that causes bat white-nose syndrome (WNS), exhibit different disease severity, viral shedding and molecular responses than bats infected with only Myl-CoV or only P. destructans. We took advantage of the natural persistence of Myl-CoV in bats that were experimentally inoculated with P. destructans in a previous study. Here, we show that the intestines of virus-infected bats that were also infected with fungus contained on average 60-fold more viral RNA than bats with virus alone. Increased viral RNA in the intestines correlated with the severity of fungus-related pathology. Additionally, the intestines of bats infected with fungus exhibited different expression of mitogen-activated protein kinase pathway and cytokine related transcripts, irrespective of viral presence. Levels of coronavirus antibodies were also higher in fungal-infected bats. Our results suggest that the systemic effects of WNS may down-regulate anti-viral responses in bats persistently infected with M. lucifugus coronavirus and increase the potential of virus shedding.",
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Davy, CM, Donaldson, ME, Subudhi, S, Rapin, N, Warnecke, L, Turner, JM, Bollinger, TK, Kyle, CJ, Dorville, NASY, Kunkel, EL, Norquay, KJO, Dzal, YA, Willis, CKR & Misra, V 2018, 'White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats', Scientific Reports, vol. 8, no. 1, 15508, pp. 1-12. https://doi.org/10.1038/s41598-018-33975-x

White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats. / Davy, Christina M.; Donaldson, Michael E.; Subudhi, Sonu; Rapin, Noreen; Warnecke, Lisa; Turner, James M.; Bollinger, Trent K.; Kyle, Christopher J.; Dorville, Nicole A.S.Y.; Kunkel, Emma L.; Norquay, Kaleigh J.O.; Dzal, Yvonne A.; Willis, Craig K.R.; Misra, Vikram.

In: Scientific Reports, Vol. 8, No. 1, 15508, 19.10.2018, p. 1-12.

Research output: Contribution to journalArticle

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AU - Davy, Christina M.

AU - Donaldson, Michael E.

AU - Subudhi, Sonu

AU - Rapin, Noreen

AU - Warnecke, Lisa

AU - Turner, James M.

AU - Bollinger, Trent K.

AU - Kyle, Christopher J.

AU - Dorville, Nicole A.S.Y.

AU - Kunkel, Emma L.

AU - Norquay, Kaleigh J.O.

AU - Dzal, Yvonne A.

AU - Willis, Craig K.R.

AU - Misra, Vikram

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Y1 - 2018/10/19

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