Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice

Panayiotis Loukopoulos, Bruce A. Mungall, Majid Ghoddusi, John R. Thornton, Wayne F. Robinson

Research output: Other contribution to conferenceAbstract


Introduction To facilitate preclinical trials, we aimed to create serial subcutaneous and lung xenotransplantation models of both primary and secondary canine osteosarcoma (COS) in mice.
Methods: Material from 6 primary osteosarcomas and 3 osteosarcoma cell lines(1) was transplanted subcutaneously and intravenously into nude mice. Xenografts were characterized employing zymography, immunohistochemistry and light and electron microscopy.
Results Tumours were successfully xenotransplanted in one case each of cultured (xenografts T1) and primary (T2) COS cells. The produced xenografts retained donor tumour characteristics, but produced low MMP levels and were self-limiting in nature. Both showed intense positive immunohistochemical staining for MMP-9 in the surrounding tissues, while the tumour cells were mostly negative. Similarly for zymography, the skin surrounding T2 produced higher MMP levels, including the 62 kDa form.
Conclusions The overall tumour intake rate was very low and the xenotransplanted tumours lacked the stability and consistency that are prerequisites for a useful in vivo model. This may be due to a range of factors, including the nature of the material transplanted, the nude mouse as the recipient species, the xenotransplantation techniques used, and the route of cell administration. To our knowledge, there are no other reports of subcutaneously xenotransplanted primary COS. Intraosseous transplantation or the use of a suitable cell substrate may increase the tumour cell intake rate in future studies.
Original languageEnglish
Number of pages1
Publication statusPublished - 2008
EventEuropean Veterinary Conference Voorjaarsdagen 2008 - Amsterdam RAI Conference Centre, Amsterdam, Netherlands
Duration: 24 Apr 200826 Apr 2008


ConferenceEuropean Veterinary Conference Voorjaarsdagen 2008
Abbreviated titleveterinary medicine
Internet address

Fingerprint Dive into the research topics of 'Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice'. Together they form a unique fingerprint.

  • Prizes

    Bequest of Brown Emily Perrett “for the benefit of animal health and welfare, including diagnosis and treatment of cancer in cats and dogs.”

    Panos Loukopoulos (Recipient), Wayne F. Robinson (Recipient) & John R. Thornton (Recipient), 1997

    Prize: Award

  • University of Queensland Sister Janet Mylonas Memorial Scholarship

    Panos Loukopoulos (Recipient), 1996

    Prize: Award

  • Cite this

    Loukopoulos, P., Mungall, B. A., Ghoddusi, M., Thornton, J. R., & Robinson, W. F. (2008). Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice. 269-269. Abstract from European Veterinary Conference Voorjaarsdagen 2008, Amsterdam, Netherlands. http://www.voorjaarsdagen.eu/conference-info/european-veterinary-conference-voorjaarsdagen/proceedings/category/32-companion-animal-posters-2008