Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice

Panayiotis Loukopoulos, Bruce A. Mungall, Majid Ghoddusi, John R. Thornton, Wayne F. Robinson

Research output: Other contribution to conferenceAbstract

Abstract

Introduction To facilitate preclinical trials, we aimed to create serial subcutaneous and lung xenotransplantation models of both primary and secondary canine osteosarcoma (COS) in mice.
Methods: Material from 6 primary osteosarcomas and 3 osteosarcoma cell lines(1) was transplanted subcutaneously and intravenously into nude mice. Xenografts were characterized employing zymography, immunohistochemistry and light and electron microscopy.
Results Tumours were successfully xenotransplanted in one case each of cultured (xenografts T1) and primary (T2) COS cells. The produced xenografts retained donor tumour characteristics, but produced low MMP levels and were self-limiting in nature. Both showed intense positive immunohistochemical staining for MMP-9 in the surrounding tissues, while the tumour cells were mostly negative. Similarly for zymography, the skin surrounding T2 produced higher MMP levels, including the 62 kDa form.
Conclusions The overall tumour intake rate was very low and the xenotransplanted tumours lacked the stability and consistency that are prerequisites for a useful in vivo model. This may be due to a range of factors, including the nature of the material transplanted, the nude mouse as the recipient species, the xenotransplantation techniques used, and the route of cell administration. To our knowledge, there are no other reports of subcutaneously xenotransplanted primary COS. Intraosseous transplantation or the use of a suitable cell substrate may increase the tumour cell intake rate in future studies.
Original languageEnglish
Pages269-269
Number of pages1
Publication statusPublished - 2008
EventEuropean Veterinary Conference Voorjaarsdagen 2008 - Amsterdam RAI Conference Centre, Amsterdam, Netherlands
Duration: 24 Apr 200826 Apr 2008
http://www.voorjaarsdagen.eu/conference-info/european-veterinary-conference-voorjaarsdagen/proceedings/category/118-voorjaarsdagen-2008

Conference

ConferenceEuropean Veterinary Conference Voorjaarsdagen 2008
Abbreviated titleveterinary medicine
CountryNetherlands
CityAmsterdam
Period24/04/0826/04/08
Internet address

Fingerprint

Heterologous Transplantation
Osteosarcoma
Nude Mice
Canidae
Matrix Metalloproteinases
Heterografts
Neoplasms
Electron Microscopy
Transplantation
Immunohistochemistry
Staining and Labeling
Light
Cell Line
Lung
Skin

Cite this

Loukopoulos, P., Mungall, B. A., Ghoddusi, M., Thornton, J. R., & Robinson, W. F. (2008). Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice. 269-269. Abstract from European Veterinary Conference Voorjaarsdagen 2008, Amsterdam, Netherlands.
Loukopoulos, Panayiotis ; Mungall, Bruce A. ; Ghoddusi, Majid ; Thornton, John R. ; Robinson, Wayne F. / Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice. Abstract from European Veterinary Conference Voorjaarsdagen 2008, Amsterdam, Netherlands.1 p.
@conference{ce50525ac81042ebada0a4c81e0ea2ef,
title = "Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice",
abstract = "Introduction To facilitate preclinical trials, we aimed to create serial subcutaneous and lung xenotransplantation models of both primary and secondary canine osteosarcoma (COS) in mice. Methods: Material from 6 primary osteosarcomas and 3 osteosarcoma cell lines(1) was transplanted subcutaneously and intravenously into nude mice. Xenografts were characterized employing zymography, immunohistochemistry and light and electron microscopy. Results Tumours were successfully xenotransplanted in one case each of cultured (xenografts T1) and primary (T2) COS cells. The produced xenografts retained donor tumour characteristics, but produced low MMP levels and were self-limiting in nature. Both showed intense positive immunohistochemical staining for MMP-9 in the surrounding tissues, while the tumour cells were mostly negative. Similarly for zymography, the skin surrounding T2 produced higher MMP levels, including the 62 kDa form. Conclusions The overall tumour intake rate was very low and the xenotransplanted tumours lacked the stability and consistency that are prerequisites for a useful in vivo model. This may be due to a range of factors, including the nature of the material transplanted, the nude mouse as the recipient species, the xenotransplantation techniques used, and the route of cell administration. To our knowledge, there are no other reports of subcutaneously xenotransplanted primary COS. Intraosseous transplantation or the use of a suitable cell substrate may increase the tumour cell intake rate in future studies.",
author = "Panayiotis Loukopoulos and Mungall, {Bruce A.} and Majid Ghoddusi and Thornton, {John R.} and Robinson, {Wayne F.}",
year = "2008",
language = "English",
pages = "269--269",
note = "European Veterinary Conference Voorjaarsdagen 2008, veterinary medicine ; Conference date: 24-04-2008 Through 26-04-2008",
url = "http://www.voorjaarsdagen.eu/conference-info/european-veterinary-conference-voorjaarsdagen/proceedings/category/118-voorjaarsdagen-2008",

}

Loukopoulos, P, Mungall, BA, Ghoddusi, M, Thornton, JR & Robinson, WF 2008, 'Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice' European Veterinary Conference Voorjaarsdagen 2008, Amsterdam, Netherlands, 24/04/08 - 26/04/08, pp. 269-269.

Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice. / Loukopoulos, Panayiotis; Mungall, Bruce A.; Ghoddusi, Majid; Thornton, John R.; Robinson, Wayne F.

2008. 269-269 Abstract from European Veterinary Conference Voorjaarsdagen 2008, Amsterdam, Netherlands.

Research output: Other contribution to conferenceAbstract

TY - CONF

T1 - Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice

AU - Loukopoulos, Panayiotis

AU - Mungall, Bruce A.

AU - Ghoddusi, Majid

AU - Thornton, John R.

AU - Robinson, Wayne F.

PY - 2008

Y1 - 2008

N2 - Introduction To facilitate preclinical trials, we aimed to create serial subcutaneous and lung xenotransplantation models of both primary and secondary canine osteosarcoma (COS) in mice. Methods: Material from 6 primary osteosarcomas and 3 osteosarcoma cell lines(1) was transplanted subcutaneously and intravenously into nude mice. Xenografts were characterized employing zymography, immunohistochemistry and light and electron microscopy. Results Tumours were successfully xenotransplanted in one case each of cultured (xenografts T1) and primary (T2) COS cells. The produced xenografts retained donor tumour characteristics, but produced low MMP levels and were self-limiting in nature. Both showed intense positive immunohistochemical staining for MMP-9 in the surrounding tissues, while the tumour cells were mostly negative. Similarly for zymography, the skin surrounding T2 produced higher MMP levels, including the 62 kDa form. Conclusions The overall tumour intake rate was very low and the xenotransplanted tumours lacked the stability and consistency that are prerequisites for a useful in vivo model. This may be due to a range of factors, including the nature of the material transplanted, the nude mouse as the recipient species, the xenotransplantation techniques used, and the route of cell administration. To our knowledge, there are no other reports of subcutaneously xenotransplanted primary COS. Intraosseous transplantation or the use of a suitable cell substrate may increase the tumour cell intake rate in future studies.

AB - Introduction To facilitate preclinical trials, we aimed to create serial subcutaneous and lung xenotransplantation models of both primary and secondary canine osteosarcoma (COS) in mice. Methods: Material from 6 primary osteosarcomas and 3 osteosarcoma cell lines(1) was transplanted subcutaneously and intravenously into nude mice. Xenografts were characterized employing zymography, immunohistochemistry and light and electron microscopy. Results Tumours were successfully xenotransplanted in one case each of cultured (xenografts T1) and primary (T2) COS cells. The produced xenografts retained donor tumour characteristics, but produced low MMP levels and were self-limiting in nature. Both showed intense positive immunohistochemical staining for MMP-9 in the surrounding tissues, while the tumour cells were mostly negative. Similarly for zymography, the skin surrounding T2 produced higher MMP levels, including the 62 kDa form. Conclusions The overall tumour intake rate was very low and the xenotransplanted tumours lacked the stability and consistency that are prerequisites for a useful in vivo model. This may be due to a range of factors, including the nature of the material transplanted, the nude mouse as the recipient species, the xenotransplantation techniques used, and the route of cell administration. To our knowledge, there are no other reports of subcutaneously xenotransplanted primary COS. Intraosseous transplantation or the use of a suitable cell substrate may increase the tumour cell intake rate in future studies.

M3 - Abstract

SP - 269

EP - 269

ER -

Loukopoulos P, Mungall BA, Ghoddusi M, Thornton JR, Robinson WF. Xenotransplantation of spontaneous primary canine osteosarcoma and cultured canine osteosarcoma cells into nude mice. 2008. Abstract from European Veterinary Conference Voorjaarsdagen 2008, Amsterdam, Netherlands.