Yeast: An overlooked component of Bactrocera tryoni (Diptera: Tephritidae) Larval Gut Microbiota

Ania T. Deutscher, Olivia L. Reynolds, Toni A Chapman

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)
    32 Downloads (Pure)

    Abstract

    Yeasts, often in hydrolyzed form, are key ingredients in the larval and adult diets of tephritid fruit fly colonies. However, very little is known about the presence or role of yeasts in the diets of tephritid fruit flies in nature. Previous studies have identified bacteria but not detected yeasts in the gut of Queensland fruit fly, Bactrocera tryoni (Froggatt), one of Australia’s most economically damaging insect pests of horticultural crops and of significant biosecurity concern domestically and internationally. Here we demonstrate that cultivable yeasts are commonly found in the gut of B. tryoni larvae from fruit hosts. Analysis of the ITS1, 5.8S rRNA gene, and ITS2 sequences of randomly selected isolates identified yeasts and yeast-like fungi of the genera Aureobasidium, Candida, Cryptococcus, Hanseniaspora, Pichia, and Starmerella. The prevalence of these yeasts in fruits suggests that larvae consume the yeasts as part of their diet. This work highlights that yeasts should be considered in future tephritid larval gut microbiota studies. Understanding tephritid–microbial symbiont interactions will lead to improvements in artificial diets and the quality of mass-reared tephritids for the sterile insect technique.

    Original languageEnglish
    Pages (from-to)298-300
    Number of pages3
    JournalJournal of Economic Entomology
    Volume110
    Issue number1
    Early online date29 Dec 2016
    DOIs
    Publication statusPublished - 2017

    Fingerprint

    Dive into the research topics of 'Yeast: An overlooked component of Bactrocera tryoni (Diptera: Tephritidae) Larval Gut Microbiota'. Together they form a unique fingerprint.

    Cite this